Refeeding

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Takeaways1
2025-01-28 20-08-19Refeeding after Prolonged Fasting for at least 3 days may induce whole-body reduction of Senescent Cells
Insights21
2025-01-30 15-39-20p16 expression is reduced in Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) in the bone marrow of 22-month-old mice at 24 hours after Refeeding following two consecutive Fasting-Mimicking Diet (FMD) cycles
2025-01-30 15-38-45p16 expression is not reduced in Short-Term Hematopoietic Stem Cells (ST-HSCs) or Multipotent Progenitors (MPPs) in the bone marrow of 22-month-old mice at 24 hours after Refeeding following two consecutive Fasting-Mimicking Diet (FMD) cycles
2025-01-30 15-06-49Undergoing a 4-day Fasting-Mimicking Diet (FMD) once every two weeks starting at 16 months of age increases the ratio of lin-Sca1+CD45- Bone Marrow Mesenchymal Stem Cells (BMMSCs) to total bone marrow cells at 7 days after Refeeding in 20.5-month-old mice compared to age-matched controls
2025-01-30 15-06-32Undergoing a 4-day Fasting-Mimicking Diet (FMD) once every two weeks starting at 16 months of age increases the ratio of BrdU+ lin-Sca1+CD45- cells to total bone marrow cells at 7 days after Refeeding following BrdU treatment in 20.5-month-old mice compared to age-matched controls
2025-01-30 15-05-43Undergoing a 4-day Fasting-Mimicking Diet (FMD) once every two weeks starting at 16 months of age increases the number of proliferating lin-Sca1+CD45- Bone Marrow Mesenchymal Stem Cells (BMMSCs) in mouse bone marrow at 7 days after Refeeding in 20.5-month-old mice compared to age-matched controls
2025-01-30 15-04-37Undergoing a 4-day Fasting-Mimicking Diet (FMD) once every two weeks starting at 16 months of age increases the number of proliferating lin-Sca1+CD45- bone marrow cells at 7 days after Refeeding in 20.5-month-old mice compared to age-matched controls
2025-01-30 15-04-10Undergoing a 4-day Fasting-Mimicking Diet (FMD) once every two weeks starting at 16 months of age increases the number of proliferating Bone Marrow Mesenchymal Stem Cells (BMMSCs) in mouse bone marrow at 7 days after Refeeding
2025-01-30 15-01-49Undergoing a 4-day Fasting-Mimicking Diet (FMD) once every two weeks starting at 16 months of age increases the number of proliferating Bone Marrow Mesenchymal Stem Cells (BMMSCs) in mouse bone marrow at 7 days after Refeeding in 20.5-month-old mice compared to age-matched controls
2025-01-30 15-00-30Undergoing a 4-day Fasting-Mimicking Diet (FMD) once every two weeks starting at 16 months of age increases the number of BrdU+ lin-Sca1+CD45- BM cells at 7 days after Refeeding following BrdU treatment in 20.5-month-old mice compared to age-matched controls
2025-01-30 14-57-31There are fewer Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) Senescent Cells in the bone marrow of mice 24 hours after Refeeding following two consecutive Fasting-Mimicking Diet (FMD) cycles
2025-01-30 14-26-56The % of cas3+p16+ Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) increases from ~1% to ~3% in the bone marrow of 22-month-old mice at 24 hours after Refeeding following two consecutive Fasting-Mimicking Diet (FMD) cycles
2025-01-30 14-24-58The % of cas3+ Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) increases from ~1% to ~3% in the bone marrow of 22-month-old mice at 24 hours after Refeeding following two consecutive Fasting-Mimicking Diet (FMD) cycles
2025-01-30 14-07-34Refeeding with ~0.4 g/kg protein after 5-day Prolonged Fasting allows serum IGF1 levels to continue decreasing when refeeding with 552-799 kcal/day (10.8 kcal/kg) but not 1797-2579 kcal/day (35.1 kcal/kg)
2025-01-30 14-05-50Refeeding causes Apoptosis in Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) Senescent Cells following two consecutive Fasting-Mimicking Diet (FMD) cycles
2025-01-30 13-54-39Refeeding after a Fasting-Mimicking Diet (FMD) causes Senescent Cells of various types throughout the body to die off
2025-01-30 13-53-46Refeeding after an Fasting-Mimicking Diet (FMD) cycle causes a reduction in Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) Senescent Cells specifically due to Apoptosis
2025-01-20 15-29-22Fasting mice for 1 or 2 days prior to muscle injury results in the same muscle Regeneration following 7 days of Refeeding
2025-01-20 15-24-25Fasting mice for 2.5 days prior to muscle injury reduces muscle Regeneration following 7 days of Refeeding compared to fasting for 2 days prior to injury
2025-01-20 15-18-43Fasting mice for 2.5 days prior to muscle injury reduces muscle Regeneration following 7 days of Refeeding compared to fasting for 1 day prior to injury
2025-01-20 15-10-32Fasting mice for as little as 1 day prior to muscle injury reduces muscle Regeneration following 7 days of Refeeding
2025-01-20 15-07-10Fasting mice for 1-2.5 days prior to muscle injury reduces muscle Regeneration following 7 days of Refeeding compared to no fasting prior to injury
Quick Notes1
2025-01-27 16-44-38Serum Myostatin stays reduced during 3 months of Refeeding after 14 days of Prolonged Fasting
Assumptions2
2025-01-28 21-32-52The reduction in IGF1 availability during a fast is responsible for the effects of Prolonged Fasting and Refeeding on Senescent Cells
2025-01-28 21-28-50The number of p16- Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) did not increase in 22-month-old mice at 24 hours after Refeeding following two consecutive Fasting-Mimicking Diet (FMD) cycles in longoUtilizationFastingMimickingDiet2023 - Figure 1D