Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs)

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Takeaways1
2025-01-28 20-11-31Prolonged Fasting for at least 9 days may induce Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) symmetric Self-Renewal
Insights24
2025-01-30 15-39-20p16 expression is reduced in Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) in the bone marrow of 22-month-old mice at 24 hours after Refeeding following two consecutive Fasting-Mimicking Diet (FMD) cycles
2025-01-30 14-57-31There are fewer Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) Senescent Cells in the bone marrow of mice 24 hours after Refeeding following two consecutive Fasting-Mimicking Diet (FMD) cycles
2025-01-30 14-56-06The total number of cycling Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) in mouse bone marrow increases by the end of 72-hour Prolonged Fasting
2025-01-30 14-54-39The total number of Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) in mouse bone marrow increases by the end of 72-hour Prolonged Fasting
2025-01-30 14-45-54The ratio of BrdU+ Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) to total bone marrow cells in mouse bone marrow is increased in mice after 72-hour Prolonged Fasting compared to non-fasting mice
2025-01-30 14-44-30The ratio of Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) to total CD45+ cells increases for LT-HSCs isolated from the BM of mice after 72-hour Prolonged Fasting compared to those of non-fasted mice upon 3-day co-culture with Lin-CD45- stromal cells from the bone marrow of 72-hour-fasted mice
2025-01-30 14-43-30The ratio of Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) to total CD45+ cells increases for LT-HSCs isolated from the bone marrow of mice after 72-hour Prolonged Fasting mice compared to those of non-fasted mice upon 3-day co-culture with Lin-CD45- stromal cells from the bone marrow of non-fasted mice
2025-01-30 14-41-07The ratio of total Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) to total bone marrow cells in mouse bone marrow is increased in mice after 72-hour Prolonged Fasting compared to non-fasting mice
2025-01-30 14-40-02The reduction in circulating IGF1 availability during fasting is responsible for the fasting-induced symmetric Self-Renewal of mouse Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) after 72-hour Prolonged Fasting
2025-01-30 14-28-44The enhanced Self-Renewal program of Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) from mice after 72-hour Prolonged Fasting is inherent to the LT-HSC cells and not related to the BM stromal niche cells
2025-01-30 14-26-56The % of cas3+p16+ Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) increases from ~1% to ~3% in the bone marrow of 22-month-old mice at 24 hours after Refeeding following two consecutive Fasting-Mimicking Diet (FMD) cycles
2025-01-30 14-25-13The % of cas3+p16+ Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) in 22-month-old mice is not affected by a Fasting-Mimicking Diet (FMD) cycle
2025-01-30 14-24-58The % of cas3+ Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) increases from ~1% to ~3% in the bone marrow of 22-month-old mice at 24 hours after Refeeding following two consecutive Fasting-Mimicking Diet (FMD) cycles
2025-01-30 14-23-59The % of cas3+ Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) in 22-month-old mice is not affected by a Fasting-Mimicking Diet (FMD) cycle
2025-01-30 14-22-58The % of mouse Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) in S-G2-M is increased at the end of 72-hour Prolonged Fasting compared with non-fasting mice
2025-01-30 14-21-44The % of mouse Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) in G1 Phase is increased at the end of 72-hour Prolonged Fasting compared with non-fasting mice
2025-01-30 14-20-22The % of mouse Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) in G0 Phase is reduced at the end of 72-hour Prolonged Fasting compared with non-fasting mice
2025-01-30 14-05-50Refeeding causes Apoptosis in Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) Senescent Cells following two consecutive Fasting-Mimicking Diet (FMD) cycles
2025-01-30 13-56-42Reducing expression of th Protein Kinase A Catalytic Subunit (PKACα) in mouse bone marrow causes proliferation of Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs)
2025-01-30 13-53-46Refeeding after an Fasting-Mimicking Diet (FMD) cycle causes a reduction in Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) Senescent Cells specifically due to Apoptosis
2025-01-30 12-05-44Injecting IGF1 into fasting mice prevented an increase in the ratio of Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) to total bone marrow cells at 72 hours of Prolonged Fasting compared with 72-hour-fasted mice that were not injected with IGF1 while fasting
2025-01-30 11-55-39Injecting IGF1 into fasting mice prevented an increase in the number of Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) at 72 hours of Prolonged Fasting compared with 72-hour-fasted mice that were not injected with IGF1 while fasting
2025-01-29 11-24-44Prolonged Fasting for 72 hours induces symmetric Self-Renewal of mouse Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs)
2025-01-29 10-57-27Ex vivo treatment of cells from mouse bone marrow with siRNA targeting the Protein Kinase A (PKA) catalytic subunit (Protein Kinase A Catalytic Subunit (PKACα)) increases the ratio of Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) to total cells compared with untreated cells
Assumptions7
2025-01-29 09-33-38The results for Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) in the HSC graph in chengProlongedFastingReduces2014 - Figure 4D are statistically significant for the PF group compared with the PF+IGF1 group
2025-01-28 21-31-35The results for Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) in the HSC graph in chengProlongedFastingReduces2014 - Figure 4D are not statistically significant for the Ctrl group compared with the PF+IGF1 group
2025-01-28 21-31-23The results for Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) in the HSC graph in chengProlongedFastingReduces2014 - Figure 2B are statistically significant for both PF cycles
2025-01-28 21-31-00The results for Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) in the HSC graph in chengProlongedFastingReduces2014 - Figure 4D are statistically significant for the PF group compared with the Ctrl group
2025-01-28 21-28-50The number of p16- Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) did not increase in 22-month-old mice at 24 hours after Refeeding following two consecutive Fasting-Mimicking Diet (FMD) cycles in longoUtilizationFastingMimickingDiet2023 - Figure 1D
2025-01-28 21-21-27The increase in Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) asymmetric Self-Renewal seen after 72 hours of Prolonged Fasting in mouse bone marrow in chengProlongedFastingReduces2014 was not triggered earlier in the fasting time
2025-01-28 21-07-52Other types of stem cells in the bone marrow (e.g. Very Small Embryonic-Like Stem Cells (VSELs)) were not responsible for the increase in Bone Marrow Long-Term Hematopoietic Stem Cells (BM LT-HSCs) after Prolonged Fasting in 72-hour-fasted mice in chengProlongedFastingReduces2014